abstract：:Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled with GFP/YFP under normal conditions and after ischemia-induced ...

journal_title：Journal of neuroscience methods

authors： Chen S,Tran S,Sigler A,Murphy TH

更新日期：2011-02-15 00:00:00

abstract：:G protein-coupled receptors constitute one of the most important families of membrane receptors through which cells respond to extracellular stimuli. Receptors of this superfamily likely function as signal transduction complexes. The identification and analysis of their components provide new insights into a better un...

journal_title：Journal of neuroscience methods

authors： Borroto-Escuela DO,Correia PA,Romero-Fernandez W,Narvaez M,Fuxe K,Ciruela F,Garriga P

更新日期：2011-02-15 00:00:00

abstract：:Benign schwannomas are common tumors of the cranial and peripheral nerves, causing pain and loss of function. The development of effective therapy for these tumors has been hampered by the lack of relevant experimental in vivo models for convenient testing. Here, we describe a novel schwannoma model in which an immort...

journal_title：Journal of neuroscience methods

authors： Saydam O,Ozdener GB,Senol O,Mizrak A,Prabhakar S,Stemmer-Rachamimov AO,Breakefield XO,Brenner GJ

更新日期：2011-01-30 00:00:00

abstract：:Functional magnetic resonance imaging (fMRI) based on BOLD signal has been used to indirectly measure the local neural activity induced by cognitive tasks or stimulation. Most fMRI data analysis is carried out using the general linear model (GLM), a statistical approach which predicts the changes in the observed BOLD ...

journal_title：Journal of neuroscience methods

authors： Lessa PS,Sato JR,Cardoso EF,Neto CG,Valadares AP,Amaro E Jr

更新日期：2011-01-15 00:00:00

abstract：:Gamma band synchronization has drawn increasing interest with respect to its potential role in neuronal encoding strategy and behavior in awake, behaving animals. However, contamination of these recordings by power line noise can confound the analysis and interpretation of cortical local field potential (LFP). Existin...

journal_title：Journal of neuroscience methods

authors： Wang Z,Roe AW

更新日期：2011-01-15 00:00:00

abstract：:MR connectomics is an emerging framework in neuro-science that combines diffusion MRI and whole brain tractography methodologies with the analytical tools of network science. In the present work we review the current methods enabling structural connectivity mapping with MRI and show how such data can be used to infer ...

journal_title：Journal of neuroscience methods

authors： Hagmann P,Cammoun L,Gigandet X,Gerhard S,Grant PE,Wedeen V,Meuli R,Thiran JP,Honey CJ,Sporns O

更新日期：2010-12-15 00:00:00

abstract：:A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...

journal_title：Journal of neuroscience methods

authors： Carrozzo MM,Cannazza G,Pinetti D,Di Viesti V,Battisti U,Braghiroli D,Parenti C,Baraldi M

更新日期：2010-12-15 00:00:00

abstract：:Chimera construction between different proteins is a useful method for investigating protein structure and function relationships. However, this technique, by its traditional application, has been daunting because of its complex procedure and low success rate. Here we describe a protocol for constructing chimeras betw...

journal_title：Journal of neuroscience methods

authors： Shan Q,Lynch JW

更新日期：2010-10-30 00:00:00

abstract：:Reach-to-grasp tasks have been used to study rodent models of motor system damage, stroke, and neurodegenerative disorders such as Parkinson's. These tasks are especially useful as they allow evaluation of bilateral or unilateral damage in different regions of the brain. Performing reach-to-grasp tests in juvenile mic...

journal_title：Journal of neuroscience methods

authors： Marques JM,Olsson IA

更新日期：2010-10-30 00:00:00

abstract：:Research findings on human motor skills may not necessarily hold in everyday life, since laboratory and everyday scenarios typically differ with respect to the subjects' attention to the skill, their motivation to perform at their best, the goals they try to achieve, and the mode of movement initiation - extrinsic ver...

journal_title：Journal of neuroscience methods

authors： Bock O,Hagemann A

更新日期：2010-10-30 00:00:00

abstract：:Mitochondrial defects have been implicated in the degeneration of axons in a number of CNS disorders, including multiple sclerosis. Uniquely, mitochondria harbor the only non-nuclear DNA (mitochondrial DNA or mtDNA), which encodes functionally important subunits of the respiratory chain. The pattern of mitochondrial r...

journal_title：Journal of neuroscience methods

authors： Zambonin J,Engeham S,Campbell GR,Ziabreva I,Beadle NE,Taylor RW,Mahad DJ

更新日期：2010-09-30 00:00:00

abstract：:Technical challenges to record cortical unit spikes in conscious behavior monkeys have been presenting constraints in the investigation of cortical control of lower limb. Here we report a novel experimental system for investigation of correlation between cortical neuronal signals and standing and squatting movement. T...

journal_title：Journal of neuroscience methods

authors： Ma C,He J

更新日期：2010-09-30 00:00:00

abstract：:Most spinal cord injury research is undertaken using in vivo animal models but the extensive care associated with spinalized animals, inherent variability between animals, and complex surgeries makes alternative models especially valuable. Here we present a novel ex vivo model that enables culture of intact post-natal...

journal_title：Journal of neuroscience methods

authors： Zhang J,O'Carroll SJ,Wu A,Nicholson LF,Green CR

更新日期：2010-09-30 00:00:00

abstract：:We report an original method for implanting bone marrow stem cells within the spinal cord parenchyma. This method was used for the experimental treatment of patients diagnosed with amyotrophic lateral sclerosis. The methodology is reproducible and devoid of major complications even in patients showing significant spin...

journal_title：Journal of neuroscience methods

authors： Blanquer M,Pérez-Espejo MA,Martínez-Lage JF,Iniesta F,Martinez S,Moraleda JM

更新日期：2010-08-30 00:00:00

abstract：:One of the challenges in neuroscience is the detection of directionality between signals reflecting neural activity. To reveal the directionality of coupling and time delays between interacting multi-scale signals, we use a combination of a data-driven technique called empirical mode decomposition (EMD) and partial di...

journal_title：Journal of neuroscience methods

authors： Silchenko AN,Adamchic I,Pawelczyk N,Hauptmann C,Maarouf M,Sturm V,Tass PA

更新日期：2010-08-15 00:00:00

abstract：:An important characterization of neural spiking is the ratio of the variance to the mean of the spike counts in a set of intervals--the Fano factor. For a Poisson process, the theoretical Fano factor is exactly one. For simulated or experimental neural data, the sample Fano factor is never exactly one, but often appea...

journal_title：Journal of neuroscience methods

authors： Eden UT,Kramer MA

更新日期：2010-06-30 00:00:00

abstract：:Generalized linear models (GLMs) have been developed for modeling and decoding population neuronal spiking activity in the motor cortex. These models provide reasonable characterizations between neural activity and motor behavior. However, they lack a description of movement-related terms which are not observed direct...

journal_title：Journal of neuroscience methods

authors： Lawhern V,Wu W,Hatsopoulos N,Paninski L

更新日期：2010-06-15 00:00:00

abstract：:Artifacts in functional magnetic resonance imaging (fMRI) data, primarily those related to motion and physiological sources, negatively impact the functional signal-to-noise ratio in fMRI studies, even after conventional fMRI preprocessing. Independent component analysis' demonstrated capacity to separate sources of n...

journal_title：Journal of neuroscience methods

authors： Kelly RE Jr,Alexopoulos GS,Wang Z,Gunning FM,Murphy CF,Morimoto SS,Kanellopoulos D,Jia Z,Lim KO,Hoptman MJ

更新日期：2010-06-15 00:00:00

abstract：:A novel epineural tube implantation paradigm in the adult rat was designed for the analysis of regulatory cell interactions in peripheral nerves and for the development of therapeutic implants. The aim was to allow the integration of synthetic regenerative structures and cells into the nerve interior while preserving ...

journal_title：Journal of neuroscience methods

authors： Hajosch R,Grupp L,Nichterwitz S,Schlosshauer B

更新日期：2010-06-15 00:00:00

abstract：:A rat model of penetrating ballistic-like brain injury (PBBI) was recently established to study military-relevant severe traumatic brain injury (TBI). The purpose of this study was to conduct a side-by-side evaluation of two well-established cognitive testing paradigms: the novel object recognition (NOR) task and the ...

journal_title：Journal of neuroscience methods

authors： Davis AR,Shear DA,Chen Z,Lu XC,Tortella FC

更新日期：2010-05-30 00:00:00

abstract：:Many neurophysiological experiments on rodents and non-human primates involve the implantation of more than one multi-electrode array to record from many regions of the brain. So called 'floating' microelectrode arrays are implanted in cortical regions of interest and are coupled via a flexible cable to their connecto...

journal_title：Journal of neuroscience methods

authors： Chhatbar PY,von Kraus LM,Semework M,Francis JT

更新日期：2010-05-15 00:00:00

abstract：:Imaging of optical reporters of neural activity across large populations of neurones is a widely used approach for investigating the function of neural circuits in slices and in vivo. Major challenges in analysing such experiments include the automatic identification of neurones and synapses, extraction of dynamic sig...

journal_title：Journal of neuroscience methods

authors： Dorostkar MM,Dreosti E,Odermatt B,Lagnado L

更新日期：2010-04-30 00:00:00

abstract：:The aim of the study was to compare saccadic peak velocity (SPV) values measured with video based Fitness Impairment Tester (FIT) and electro-oculography (EOG) during prolonged wakefulness. We tested different numbers of saccades and two saccade paradigms to improve the EOG measurements for detecting fatigue. The SPVs...

journal_title：Journal of neuroscience methods

authors： Hirvonen K,Puttonen S,Gould K,Korpela J,Koefoed VF,Müller K

更新日期：2010-03-30 00:00:00

abstract：:Isolated turtle brain/eye preparation has recently been used as a bloodless animal model for detecting the magnetic resonance imaging (MRI) signal changes produced by visually evoked neuronal currents. The present work aims to determine whether checkerboard-patterned or full field flash (blank) stimulation should be u...

journal_title：Journal of neuroscience methods

authors： Luo Q,Lu H,Lu H,Yang Y,Gao JH

更新日期：2010-03-15 00:00:00

abstract：:The present work introduces an electrode microdrive system small enough to be placed into two distinct brain areas in head-fixed mice. To meet the space constraint imposed by the size of mice and the additional presence of a head post, the size and weight of the components were minimized. In the current version, an in...

journal_title：Journal of neuroscience methods

authors： Haiss F,Butovas S,Schwarz C

更新日期：2010-03-15 00:00:00

abstract：:Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be use...

journal_title：Journal of neuroscience methods

authors： Lu CM,Zhang YJ,Biswal BB,Zang YF,Peng DL,Zhu CZ

更新日期：2010-02-15 00:00:00

abstract：:The use of drugs for recreational purposes, in particular Methamphetamine, is associated with an increased risk of infection with human immunodeficiency virus (HIV)-1. HIV-1 infection in turn can lead to HIV-associated neurological disorders (HAND) that range from mild cognitive and motor impairment to HIV-associated ...

journal_title：Journal of neuroscience methods

authors： Roberts AJ,Maung R,Sejbuk NE,Ake C,Kaul M

更新日期：2010-02-15 00:00:00

abstract：:Ferrets have been extensively used to unravel the neural mechanisms of coding and processing of visual information, and also to identify the developmental mechanisms underlying the emergence of such a complex and fine-tuned neural system. In recent years numerous tools have been generated that allow studying neural sy...

journal_title：Journal of neuroscience methods

authors： Borrell V

更新日期：2010-02-15 00:00:00

abstract：:The neuromuscular junction (NMJ) in Drosophila larvae serves as an important model system to study synaptic vesicle recycling, plasticity, development, and organelle trafficking in nerve terminals. Here we provide a protocol that reliably localizes proteins subcellularly in this system in combination with good preserv...

journal_title：Journal of neuroscience methods

authors： Jiao W,Shupliakov A,Shupliakov O

更新日期：2010-01-15 00:00:00

abstract：:Activity-dependent bulk endocytosis (ADBE) is the dominant synaptic vesicle (SV) retrieval mode in central nerve terminals during periods of intense neuronal activity. Despite this fact there are very few real time assays that report the activity of this critical SV retrieval mode. In this paper we report a simple and...

journal_title：Journal of neuroscience methods

authors： Clayton EL,Cousin MA

更新日期：2009-12-15 00:00:00

abstract：:Cell transplantation therapy has raised a great interest in the perspective of its employment for nerve tissue repair. Among the various cell populations proposed, olfactory ensheathing glial cells have raised great interest over recent years, especially in the perspective of their employment for neural repair because...

journal_title：Journal of neuroscience methods

authors： Audisio C,Raimondo S,Nicolino S,Gambarotta G,Di Scipio F,Macrì L,Montarolo F,Giacobini-Robecchi MG,Porporato P,Filigheddu N,Graziani A,Geuna S,Perroteau I

更新日期：2009-12-15 00:00:00

abstract：:In this paper we present an efficient method to generate realistic simulations of extracellular recordings. The method uses a hybrid and computationally simple approach, where the features of the background noise arise naturally from its biophysical process of generation. The generated data resemble the characteristic...

journal_title：Journal of neuroscience methods

authors： Martinez J,Pedreira C,Ison MJ,Quian Quiroga R

更新日期：2009-11-15 00:00:00

abstract：:Cytochrome c oxidase or mitochondrial respiratory chain complex IV is where over 90% of oxygen is consumed. The relationship between complex IV activity and mitochondrial proteins, which provides a guide to understanding the mechanisms in primary mitochondrial disorders, has been determined by histochemistry (activity...

journal_title：Journal of neuroscience methods

authors： Mahad DJ,Ziabreva I,Campbell G,Laulund F,Murphy JL,Reeve AK,Greaves L,Smith KJ,Turnbull DM

更新日期：2009-11-15 00:00:00

abstract：:Vibrotactile stimulation has been used successfully to activate the human somatosensory pathway in functional magnetic resonance imaging (fMRI) experiments. The design and characterization of these devices are of particular interest in frequency discrimination tasks and investigations of the somatopic organization of ...

journal_title：Journal of neuroscience methods

authors： Chakravarty MM,Broadbent S,Rosa-Neto P,Lambert CM,Collins DL

更新日期：2009-10-30 00:00:00

abstract：:Sensory neurons encode external information by a series of times of action potentials, which is called a spike train. However, since it is a point process, it is hard to analyze. Here we propose a method for converting a spike train into a real-valued time series with a fixed sampling interval under the assumption of ...

journal_title：Journal of neuroscience methods

authors： Hirata Y,Aihara K

更新日期：2009-10-15 00:00:00

abstract：:The chronic intrathecal catheterization of the subarachnoid space in the rat is a very useful model in the research of neuroscience, anesthesiology, neurotoxicity, and etc. To avoid high postoperative mortality and neurological impairment, the catheterization from the lumbar interspace has been widely used in recent y...

journal_title：Journal of neuroscience methods

authors： Xu F,Li T,Zhang B

更新日期：2009-10-15 00:00:00

abstract：:We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...

journal_title：Journal of neuroscience methods

authors： Purcell EK,Thompson DE,Ludwig KA,Kipke DR

更新日期：2009-10-15 00:00:00

abstract：:Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...

journal_title：Journal of neuroscience methods

authors： Knock SA,McIntosh AR,Sporns O,Kötter R,Hagmann P,Jirsa VK

更新日期：2009-09-30 00:00:00

abstract：:We investigate the mode locking properties of simple dynamical models of pulse-coupled neurons to two tones, i.e., simple musical intervals. A recently proposed nonlinear synchronization theory of musical consonance links the subjective ranking from consonant to dissonant intervals to the universal ordering of robustn...

journal_title：Journal of neuroscience methods

authors： Heffernan B,Longtin A

更新日期：2009-09-30 00:00:00

abstract：:In order to improve cell therapy techniques, we have characterized a multipotent neural precursor cell isolation technique from the subventricular zone of adult pig brain. The pig is a non-primate species that is immunologically closest to human. The proliferative zone of this neurogenic structure was first localized ...

journal_title：Journal of neuroscience methods

authors： Liard O,Segura S,Pascual A,Gaudreau P,Fusai T,Moyse E

更新日期：2009-09-15 00:00:00